Binding of [18F]-7B with Aβ1-42 fibrils and AD homogenates. AD homogenates (A, B) and Aβ1-42
fibrils (C, D) were incubated with increasing concentrations of [18F]-7B. Nonspecific binding was determined in parallel experiments utilizing 7A (5 μM) as a competitor. Representative plots of specific binding versus [18F]-7B concentration are shown for AD homogenates in (A) and Aβ fibrils in (C). Data points represent mean ± standard deviations (n = 3). The data were analyzed by curve fitting to a one-site binding model using a nonlinear regression. Scatchard plots of the binding data are shown for AD homogenates (B) and Aβ1-42 fibrils (D). Similar results were obtained in more than three independent experiments.