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Table 2 Primary antibodies and methods used for immunohistochemistry

From: Somatostatin receptor subtype 2 in high-grade gliomas: PET/CT with 68Ga-DOTA-peptides, correlation to prognostic markers, and implications for targeted radiotherapy

Antibody Clone Manufacturer Method Detection
SSTR2 UMB-1 Abcam, Cambridge, UK Ventana Benchmark XT Autostainer (Ventana Medical Systems, Strasbourg, France) ultraVIEW Universal Detection Kit (Ventana, Strasbourg, France)
SSTR3 Rabbit polyclonal (ab28680) Abcam, Cambridge, UK Labvision Autostainer (Thermo Scientific Inc, Kalamazoo, MI) BrightVision Detection Kit (Immunologic, Duiven, the Netherlands)
SSTR5 Rabbit polyclonal (AB5681) Millipore, Billerica, MA Labvision Autostainer BrightVision Detection Kit
EGFR 5B7 Ventana, Strasbourg, France Ventana Benchmark XT Autostainer ultraVIEW Universal Detection Kit
IDH1 R132H H09 Dianova, Hamburg, Germany Ventana Benchmark XT Autostainer ultraVIEW Universal Detection Kit
CD68 PG-M1 Dako, Glostrup, Denmark Ventana Benchmark XT Autostainera ultraVIEW Universal Detection Kit + Universal Alkaline Phosphatase Red Detection Kit (Ventana)a
Ki67 30-9 Ventana, Strasbourg, France Ventana Benchmark XT Autostainer ultraVIEW Universal Detection kit
  1. aSSTR2 + CD68 double staining.
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