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Table 2 Primary antibodies and methods used for immunohistochemistry

From: Somatostatin receptor subtype 2 in high-grade gliomas: PET/CT with 68Ga-DOTA-peptides, correlation to prognostic markers, and implications for targeted radiotherapy

Antibody

Clone

Manufacturer

Method

Detection

SSTR2

UMB-1

Abcam, Cambridge, UK

Ventana Benchmark XT Autostainer (Ventana Medical Systems, Strasbourg, France)

ultraVIEW Universal Detection Kit (Ventana, Strasbourg, France)

SSTR3

Rabbit polyclonal (ab28680)

Abcam, Cambridge, UK

Labvision Autostainer (Thermo Scientific Inc, Kalamazoo, MI)

BrightVision Detection Kit (Immunologic, Duiven, the Netherlands)

SSTR5

Rabbit polyclonal (AB5681)

Millipore, Billerica, MA

Labvision Autostainer

BrightVision Detection Kit

EGFR

5B7

Ventana, Strasbourg, France

Ventana Benchmark XT Autostainer

ultraVIEW Universal Detection Kit

IDH1 R132H

H09

Dianova, Hamburg, Germany

Ventana Benchmark XT Autostainer

ultraVIEW Universal Detection Kit

CD68

PG-M1

Dako, Glostrup, Denmark

Ventana Benchmark XT Autostainera

ultraVIEW Universal Detection Kit + Universal Alkaline Phosphatase Red Detection Kit (Ventana)a

Ki67

30-9

Ventana, Strasbourg, France

Ventana Benchmark XT Autostainer

ultraVIEW Universal Detection kit

  1. aSSTR2 + CD68 double staining.