Figure 3
Fluorescence and radionuclide cell uptake studies. (a) ASP+-guided flow cytometry assessing hNET function in SupT1/NT (white), SupT1/FLuc.2A.RQR8.2A.hNET (beige), SupT1/hNET.2A.FLuc.2A.RQR8 (light grey) and SupT1/hNET.l.dCD34 (dark grey) sorted cells. Mean fluorescence intensities under the curves are presented. (b) hNET function in SupT1/hNET.2A.FLuc.2A.RQR8 cells was further characterised with an 125I-MIBG radiosubstrate uptake assay. Percent uptake was determined for SupT1/NT (white), SupT1/hNET.2A.FLuc.2A.RQR8 (light grey) and SupT1/hNET.l.dCD34 (dark grey) cells via gamma counting. Error bars are the mean ± SD for n = 3. Stats tests performed were ANOVA and Tukey’s HSD post hoc.