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EJNMMI Research
Figure 2 | EJNMMI Research

Figure 2

From: Fluorescence-guided development of a tricistronic vector encoding bimodal optical and nuclear genetic reporters for in vivo cellular imaging

Figure 2

Tricistronic hNET vector design and ASP + -guided FACS. Three hNET encoding vectors were designed and transduced into SupT1 cells; hNET.l.dCD34 (vector 1), SupT1/FLuc.2A.RQR8.2A.hNET (vector 2), SupT1/hNET.2A.FLuc.2A.RQR8 (vector 3). Transduced (grey lines) and non-transduced (blue lines) cell populations were stained with ASP+ followed by flow cytometry (left column). ASP+-guided FACS was performed by gating on the brightest 5% within each of the three cell populations (right column).

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