Tricistronic hNET vector design and ASP
-guided FACS. Three hNET encoding vectors were designed and transduced into SupT1 cells; hNET.l.dCD34 (vector 1), SupT1/FLuc.2A.RQR8.2A.hNET (vector 2), SupT1/hNET.2A.FLuc.2A.RQR8 (vector 3). Transduced (grey lines) and non-transduced (blue lines) cell populations were stained with ASP+ followed by flow cytometry (left column). ASP+-guided FACS was performed by gating on the brightest 5% within each of the three cell populations (right column).