Figure 2
From: [18F]FDG-6-P as a novel in vivo tool for imaging staphylococcal infections
Uptake of [ 18 F]FDG-6-P and [ 18 F]FDG by S. aureus and mammalian cells. (a) S. aureus RN6390 and the isogenic UHPT-deficient mutant were incubated with either [18F]FDG or [18F]FDG-6-P. The activity associated with each of the strains after incubation with [18F]FDG (black) or [18F]FDG-6-P (white) was measured using a gamma counter. Counts were normalised to remove ‘no cell’ control counts and to compare [18F]FDG-6-P uptake with [18F]FDG uptake. Error bars show SEM. **P = 0.0015; ****P < 0.0001. Data were collected from three independent repeats. (b) The activity associated with mammalian cell lines HL60, Jurkat, AGS, THP1, HIB-1B and 3 T3-L1 after incubation with [18F]FDG (black) or [18F]FDG-6-P (white) was measured using a gamma counter. The counts were normalised to compare [18F]FDG-6-P uptake with [18F]FDG uptake. Error bars show SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Differences in the means of cells incubated with [18F]FDG-6-P were not significant.