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Table 2 Results of iodination of cetuximab with increasing amount of 125 I

From: The ultimate radiochemical nightmare: upon radio-iodination of Botulinum neurotoxin A, the introduced iodine atom itself seems to be fatal for the bioactivity of this macromolecule

Cetuximab (μg) 125 I (MBq) Labelling efficiency (%) Radiochemical purity (%) Immunoreactivity (%) 125 I/mAb molar ratio a
50 15.0 71.4 99.1 95.2 0.40
50 48.4 75.2 99.7 ND 1.36
50 73.1 81.9 99.3 96.9 2.29
50 112.2 72.9 99.8 ND 3.05
50 150.0 77.0 99.4 96.8 4.30
  1. Conditions: 2.5 μg IODOGEN, 250 μL reaction volume and 90-s reaction time. ND = not determined.
  2. a100 MBq 125I corresponds with 1.24 nmol I atoms.