Molecular imaging of angiogenesis after myocardial infarction by 111In-DTPA-cNGR and 99mTc-sestamibi dual-isotope myocardial SPECT

EJNMMI Research

Table 1 Overview of the number of animals that were subjected to different SPECT scans

Scan	Scan protocol	Injected activity (MBq)	Number of Swiss mice used
In vivo; MI mice	8 consecutive time frames (15 min each)	30 to 100 MBq ¹¹¹In-DTPA-cNGR co-injected with 100 to 150 MBq ^99mTc-sestamibi	7
In vivo; control mice	8 consecutive time frames (15 min each)	30 to 100 MBq ¹¹¹In-DTPA-cNGR co-injected with 100 to 150 MBq ^99mTc-sestamibi	5
Ex vivo; heart slices of MI mice	1 time frame (240 min)	-	Out of 7 MI mice, 4 were used for ex vivo scanning purposes
Ex vivo; explanted Matrigels	1 time frame (180 min)	90 to 110 MBq ¹¹¹In-DTPA-cNGR	6^a

^aSix Swiss mice were implanted bilaterally subcutaneously with Matrigels (hFGF2-supplemented or PBS); hence, six pairs of Matrigels were scanned.