Figure 2
From: Immuno-PET of epithelial ovarian cancer: harnessing the potential of CA125 for non-invasive imaging
Confocal microscopy and flow cytometry. (A) Confocal microscopy images from immunofluorescence studies. (a-b) Immunostaining of NIH:OVCAR-3 cells with unlabeled versus FITC-labeled anti-CA125 MAb. Goat anti-mouse Alexa Fluor 488 IgG (GAM-A488) was used as secondary antibody. (c-d) Immunostaining of NIH:OVCAR-3 cells with unlabeled versus FITC-labeled anti-CA125 scFv. Penta-His Alexa Fluor 488 antibody was used as secondary antibody. (e) Unstained NIH:OVCAR-3 cells - blank. (f) NIH:OVCAR-3 cells stained with GAM-A488 antibody - negative control. (g) NIH:OVCAR-3 cells stained with Penta-His Alexa Fluor 488 antibody - negative control. (h) Immunostaining of NIH:OVCAR-3 cells with MAb 12A1 and GAM-A488 antibody - isotype control. (i-j) Immunostaining of SKOV3 cells with unlabeled versus FITC-labeled anti-CA125 MAb. (k-l) Immunostaining of SKOV3 cells with unlabeled versus FITC-labeled anti-CA125 scFv. Scale bar represents 10 μm. Flow cytometry analysis of (B) anti-CA125 MAb and (C) anti-CA125 scFv binding to NIH:OVCAR-3 cells.