Figure 2

Semi-preparative RP-HPLC for the isolation of [¹⁸F ]NS14490 in a module system with different columns. Multospher column (left; 35% MeCN, 65% water, 12.5 mM ammonium acetate (NH₄OAc), flow rate 2.5 mL min⁻¹), a Reprosil-Pur C₁₈ AQ column (right; 35% MeCN, 65% water, 12.5 mM NH₄OAc, flow rate 2 to 3 mL min⁻¹) and a Reprosil-Gold column (bottom; 35% MeCN, 65% water, 20 mM NH₄OAc, flow rate 2 mL min⁻¹).