Modification of intracellular glutathione status does not change the cardiac trapping of 64Cu(ATSM)

EJNMMI Research

Table 1 Effect of GSH concentration on the fast and slow clearance rates of ⁶⁴ Cu(ATSM)

Clearance rate	Type of heart	Injection	Untreated	BSO	NAC
FCR (k_d (min⁻¹))	Normoxic hearts	Normoxia	1.2 ± 0.2	1.1 ± 0.1	1.3 ± 0.2
		Normoxia	1.1 ± 0.2	1.1 ± 0.2	1.4 ± 0.1
		Normoxia	1.2 ± 0.1	1.2 ± 0.2	1.4 ± 0.4
	Hypoxic hearts	Normoxia	1.2 ± 0.2	1.1 ± 0.2	1.2 ± 0.2
		Early hypoxia	1.9 ± 0.4	1.8 ± 0.5	1.9 ± 0.3
		Late hypoxia	2.5 ± 0.6*	2.2 ± 0.5*	2.2 ± 0.8*
SCR (k_b (min⁻¹))	Normoxic hearts	Normoxia	0.01. ± 0.002	0.008 ± 0.002	0.01 ± 0.006
		Normoxia	0.01 ± 0.002	0.01 ± 0.008	0.01 ± 0.006
		Normoxia	0.01 ± 0.002	0.01 ± 0.004	0.01 ± 0.004
	Hypoxic hearts	Normoxia	0.01 ± 0.007	0.01 ± 0.002	0.01 ± 0.002
		Early hypoxia	0.007 ± 0.005	0.008 ± 0.005	0.009 ± 0.002
		Late hypoxia	0.003 ± 0.003*	0.004 ± 0.002*	0.005 ± 0.002*

The effect of GSH supplementation (with NAC) and GSH depletion (with BSO) upon the fast clearance rate (FCR) and slow clearance rate (SCR) of ⁶⁴Cu(ATSM) in normoxic and hypoxic hearts. Data represent means ± SD, n = 5 per group, *p < 0.05 vs. normoxic equivalent.