In vitro binding of [18F]flutemetamol to Aβ deposits in the brain of mouse models of AD. Autoradiographs of brain sections from transgenic (TG) APP23 (21 months), Tg2576 (22 months) and APPswe-PS1dE9 (19 months) transgenic mice show [18F]flutemetamol binding to whole sections (left panels) and adjacent sections treated with 10 μM cold PIB (+10 μM PIB) (right panels). High magnifications of areas enclosed in boxes show Aβ deposits detected with [18F]flutemetamol autoradiography (ARG, left column), compared to the same section stained with Thioflavin S (ThS, middle column), and an adjacent section stained with an anti-Aβ antibody (Aβ1-40, right column). Scale bar = 200 μm. mo, age of animal in months.