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Figure 1 | EJNMMI Research

Figure 1

From: Optimising the radiolabelling properties of technetium tricarbonyl and His-tagged proteins

Figure 1

TLC quality control of [99mTcO 4 ]-reduction to [99mTc(CO) 3 ]+. Rf = 0.9 for [99mTcO4]- (green arrow), Rf = 0 for 99mTc colloids (blue arrow) and Rf = 0.2 to 0.8 for [99mTc(CO)3]+ (red arrow). (a) Commercial IsoLink kit with 1 mL [99mTcO4]- (method 1), (b) modified IsoLink kit with 350 μL [99mTcO4]- (method 2) and (c) adapted kit with 100 μL [99mTcO4]- (method 3). (d) Commercial IsoLink kit dissolved with 260 μL [99mTcO4]-. Kits (a, b, c) result in >98% radiochemical yield for [99mTc(CO)3]+ formation, whereas kit (d) resulted in 82% ± 2.9% radiochemical yield for [99mTc(CO)3]+ formation.

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