Figure 4

Dual immunofluorescent staining for cells expressing RAGE in ischemic limb sections. Sites of RAGE expression were shown to be mainly myocytes based on co-localization of RAGE (Texas Red) with anti-sarcomeric actin (green, fluorescein isothiocyanate) in the merged image. Co-localization of RAGE with macrophages (Mac-3, fluorescein isothiocyanate) was also seen in the merged image. Areas in yellow represent co-localization. EC, endothelial cells; Mac, macrophages.