Figure 1From: Multimodality imaging using SPECT/CT and MRI and ligand functionalized 99mTc-labeled magnetic microbubbles Production of MBs and quality control. The MBs were washed in several steps (part I, A). MBs in the upper phase were separated by centrifugation, isolating the plain MBs with a positively charged surface. These MBs were then functionalized by different ligands using the layer-by-layer technique (part I, B). As quality control, DTPA-SPION MBs were visualized by the confocal laser microscope (Leica, resolution 550 μm × 550 μm; part II), and flow cytometry (Partec CyFlow ML flow cytometer) was used to determine the size distribution of DTPA-MBs (part III) using forward scattering versus side scattering dot plot.Back to article page