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Table 1 Analytical data for NOTA-BBN(6-14) monomer and dimmers

From: Comparative study of 64Cu/NOTA-[D-Tyr6,βAla11,Thi13,Nle14]BBN(6-14) monomer and dimers for prostate cancer PET imaging

Peptide [M] + Yield Purity b Ki c Labeling
  Calcd Found a (%) (%) (nM) yield d (%)
Bombesin      0.59 ± 0.32  
NOTA-monomer 1,570 1,571 38 99 2.51 ± 1.54e > 95
South BBN peptide segment 1,879 1,880   84f   
NOTA-dimer 1 2,976 2,976 28 99 2.00 ± 1.59 > 95
NOTA-dimer 2 3,122 3,122 31 99 1.76 ± 1.30 > 95
  1. aMass values were obtained by MALDI TOF mass spectroscopy or LC/MS/MS. bPurity was determined by HPLC analysis. cAffinities for GRPR were determined with [125I-Tyr4]bombesin in PC3 human prostate cancer cell line. dLabeling yield (not decay-corrected) was determined by radio-HPLC analysis based on 64Cu starting activity. eSimilar Ki values were obtained for the free chelate (1.30 ± 0.74) and the 'cold' Cu/NOTA-monomer (1.60 ± 0.59) when tested in triplicate in human breast cancer T47D cells. fHPLC yield of the crude peptide. NOTA, 1,4,7-triazacyclononane-1,4,7-triacetic acid; Ki, inhibition constant.