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Table 1 Analytical data for NOTA-BBN(6-14) monomer and dimmers

From: Comparative study of 64Cu/NOTA-[D-Tyr6,βAla11,Thi13,Nle14]BBN(6-14) monomer and dimers for prostate cancer PET imaging

Peptide

[M] +

Yield

Purity b

Ki c

Labeling

 

Calcd

Found a

(%)

(%)

(nM)

yield d (%)

Bombesin

    

0.59 ± 0.32

 

NOTA-monomer

1,570

1,571

38

99

2.51 ± 1.54e

> 95

South BBN peptide segment

1,879

1,880

 

84f

  

NOTA-dimer 1

2,976

2,976

28

99

2.00 ± 1.59

> 95

NOTA-dimer 2

3,122

3,122

31

99

1.76 ± 1.30

> 95

  1. aMass values were obtained by MALDI TOF mass spectroscopy or LC/MS/MS. bPurity was determined by HPLC analysis. cAffinities for GRPR were determined with [125I-Tyr4]bombesin in PC3 human prostate cancer cell line. dLabeling yield (not decay-corrected) was determined by radio-HPLC analysis based on 64Cu starting activity. eSimilar Ki values were obtained for the free chelate (1.30 ± 0.74) and the 'cold' Cu/NOTA-monomer (1.60 ± 0.59) when tested in triplicate in human breast cancer T47D cells. fHPLC yield of the crude peptide. NOTA, 1,4,7-triazacyclononane-1,4,7-triacetic acid; Ki, inhibition constant.