I]SGMIB-MNT binding/uptake and washout kinetics. Kinetics of binding and accumulation of [125I]SGMIB-MNT by (a) A431 human epidermoid carcinoma cells and (b) D247 MG human glioma cells. Total activity accumulated inside the cells (circles); total membrane-bound activity (squares). Washout kinetics of intracellular accumulated after 24 h of incubation [125I]SGMIB-MNT by (c) A431 human epidermoid carcinoma cells and (d) D247 MG human glioma cells. All data are expressed as activity in CPM per cell. For each data point, the experiment was done in triplicate with bars representing the standard error of mean.