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Table 2 Influence of experimental parameters on tissue distribution of 18 F-FDG or 18 F-fallypride expressed as normal %ID/g (mean ± SD)

From: Impact of inherent variability and experimental parameters on the reliability of small animal PET data

Part Experimental parameter Organ
A Anesthetic protocol (18F-FDG) Brain Muscle Blood  
   Control 0.31 ± 0.04 0.066 ± 0.026 0.015 ± 0.006  
   Dynamic 0.19 ± 0.04** 0.028 ± 0.006** 0.051 ± 0.009**  
   Static 0.26 ± 0.02** 0.04 ± 0.006** 0.033 ± 0.004**  
B Ambient temperature/10% EtOH (18F-FDG) Brown fat tissue Muscle   
   r.t. (23°C) 0.30 ± 0.18 0.097 ± 0.038   
   33°C 0.18 ± 0.07 (n.s.) 0.119 ±0.016*   
   r.t./10% EtOH 0.37 ± 0.14 0.08 ±0.023*   
   33°C/10% EtOH 0.40 ± 0.19 0.089 ± 0.018   
C 10% EtOH (18F-fallypride) Striatum    
   r.t. (23°C) 0.25 ± 0.03 (n.s.)    
   33°C -    
   r.t./10% EtOH 0.28 ± 0.03 (n.s.)    
   33°C/10% EtOH -    
D Gender (18F-FDG) Liver Blood Fat Reproductive organs
   Female 0.018 ± 0.005 0.01 ± 0.003 0.024 ± 0.015 0.052 ± 0.017
   Male 0.022 ± 0.006* 0.015 ± 0.005** 0.011 ± 0.009** 0.041 ± 0.009**
E Age (18F-FDG) Bone Harderian gland Urine  
   5 weeks 0.097 ± 0.016 0.45 ± 0.12 3.4 ± 2.3  
   14 weeks 0.084 ± 0.022* 0.63 ± 0.22** 1.9 ± 1.1*  
  1. n.s., Not significant. Part A is the influence of anesthesia duration on 18F-FDG distribution. **P < 0.01 for comparison with control condition. Ex vivo biodistribution of 18F-FDG in Crl:CD(SD) rats (male), n = 6. Control: anesthesia from 55 to 61 min p.i.; dynamic: anesthesia from 0 to 61 min p.i.; static: anesthesia from 20 to 61 min p.i. Part B is the influence of ambient temperature and ethanol application on 18F-FDG distribution. *P < 0.05 for comparison between conditions in bold. Ex vivo biodistribution of 18F-FDG in NMRI mice (male) under control conditions, ambient temperature control between 0 to 20 min p.i., 10% ethanol in the administered tracer solution (10 μL ethanol) and a combination of the two; n = 8. Part C is the influence of ethanol in the tracer solution on 18F-fallypride distribution. Ex vivo biodistribution of 18F-fallypride in C57Bl/6 J mice (male), n = 8. Comparison of mice receiving 10% ethanol in the administered tracer solution (corresponding to 10 μL ethanol) with control animals. Part D is the influence of gender, age, and cage occupancy on 18F-FDG biodistribution. *P < 0.05, **P < 0.01. ex vivo biodistribution of 18F-FDG in male and female NMRI mice (n = 38 for males, n = 44 for females). Part E is the ex vivo biodistribution of 18F-FDG in 14 weeks and 5 weeks old NMRI mice (n = 40 for ‘old’ animals and n = 42 for ‘young’ animals). *P < 0.05, **P < 0.01 for comparison between male and female or 5 and 14 weeks old animals, respectively.