Impact of inherent variability and experimental parameters on the reliability of small animal PET data

Martic-Kehl, Marianne Isabelle; Ametamey, Simon Mensah; Alf, Malte Frederick; Schubiger, Pius August; Honer, Michael

doi:10.1186/2191-219X-2-26

EJNMMI Research

Table 2 Influence of experimental parameters on tissue distribution of ¹⁸ F-FDG or ¹⁸ F-fallypride expressed as normal %ID/g (mean ± SD)

From: Impact of inherent variability and experimental parameters on the reliability of small animal PET data

Part	Experimental parameter	Organ
A	Anesthetic protocol (¹⁸F-FDG)	Brain	Muscle	Blood
	Control	0.31 ± 0.04	0.066 ± 0.026	0.015 ± 0.006
	Dynamic	0.19 ± 0.04**	0.028 ± 0.006**	0.051 ± 0.009**
	Static	0.26 ± 0.02**	0.04 ± 0.006**	0.033 ± 0.004**
B	Ambient temperature/10% EtOH (¹⁸F-FDG)	Brown fat tissue	Muscle
	r.t. (23°C)	0.30 ± 0.18	0.097 ± 0.038
	33°C	0.18 ± 0.07 (n.s.)	0.119 ± 0.016*
	r.t./10% EtOH	0.37 ± 0.14	0.08 ± 0.023*
	33°C/10% EtOH	0.40 ± 0.19	0.089 ± 0.018
C	10% EtOH (¹⁸F-fallypride)	Striatum
	r.t. (23°C)	0.25 ± 0.03 (n.s.)
	33°C	-
	r.t./10% EtOH	0.28 ± 0.03 (n.s.)
	33°C/10% EtOH	-
D	Gender (¹⁸F-FDG)	Liver	Blood	Fat	Reproductive organs
	Female	0.018 ± 0.005	0.01 ± 0.003	0.024 ± 0.015	0.052 ± 0.017
	Male	0.022 ± 0.006*	0.015 ± 0.005**	0.011 ± 0.009**	0.041 ± 0.009**
E	Age (¹⁸F-FDG)	Bone	Harderian gland	Urine
	5 weeks	0.097 ± 0.016	0.45 ± 0.12	3.4 ± 2.3
	14 weeks	0.084 ± 0.022*	0.63 ± 0.22**	1.9 ± 1.1*

n.s., Not significant. Part A is the influence of anesthesia duration on ¹⁸F-FDG distribution. **P < 0.01 for comparison with control condition. Ex vivo biodistribution of ¹⁸F-FDG in Crl:CD(SD) rats (male), n = 6. Control: anesthesia from 55 to 61 min p.i.; dynamic: anesthesia from 0 to 61 min p.i.; static: anesthesia from 20 to 61 min p.i. Part B is the influence of ambient temperature and ethanol application on ¹⁸F-FDG distribution. *P < 0.05 for comparison between conditions in bold. Ex vivo biodistribution of ¹⁸F-FDG in NMRI mice (male) under control conditions, ambient temperature control between 0 to 20 min p.i., 10% ethanol in the administered tracer solution (10 μL ethanol) and a combination of the two; n = 8. Part C is the influence of ethanol in the tracer solution on ¹⁸F-fallypride distribution. Ex vivo biodistribution of ¹⁸F-fallypride in C57Bl/6 J mice (male), n = 8. Comparison of mice receiving 10% ethanol in the administered tracer solution (corresponding to 10 μL ethanol) with control animals. Part D is the influence of gender, age, and cage occupancy on ¹⁸F-FDG biodistribution. *P < 0.05, **P < 0.01. ex vivo biodistribution of ¹⁸F-FDG in male and female NMRI mice (n = 38 for males, n = 44 for females). Part E is the ex vivo biodistribution of ¹⁸F-FDG in 14 weeks and 5 weeks old NMRI mice (n = 40 for ‘old’ animals and n = 42 for ‘young’ animals). *P < 0.05, **P < 0.01 for comparison between male and female or 5 and 14 weeks old animals, respectively.

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