Figure 5
Comparative in vivo stability of [111 In]AT1S and [111 In]AT2S. Analysis by HPLC of murine blood collected 5 min after injection of (A) [111In]AT1S and (B) [111In]AT2S. Co-injection with (C) [111In]AT1S and (D) [111In]AT2S radioligands show the t R of the respective intact radiopeptides. Analyses were performed on a Waters 600 HPLC system with a Symmetry Shield RP18 column eluted at a flow rate of 1 mL/min with a linear gradient of 0% B to 50% B in 50 min, with A = 0.1% TFA (v/v) and B = MeCN; the position of original peptide elution is indicated by the arrow, and the number by the arrow shows the percentage (%) of intact peptide detected in the blood.