receptor internalization in HEK-sst
determined by immunofluorescence microscopy. HEK-sst2
(A) or HEK-sst3
(B) cells were treated for 30 min at 37 °C with increasing concentrations ranging between 1 and 100 nM of either AT2S or SS14 (positive control) and then processed for immunofluorescence microscopy. AT2S is an agonist at sst2 and sst3 since it stimulates internalization of both receptors.