Coomassie stained SDS-PAGE of anxA5-DTPA (6). (A) The lanes show cys-anxA5, 5 (a) and M1234-anxA5 (b) in comparison to M1234-anxA5-DTPA (c) and anxA5-DTPA 6 (d) (both synthesised with eightfold excess of linker 4 in step 1). Lanes c and d show M1234-anxA5-DTPA and anxA5-DTPA 6 plus unreacted M1234-cys-anxA5 and cys-anxA5, 5. Note: No or minimal protein-linker dimers (from linker dimer 3 and cys-anxA5, 5) are visible. (B) Gel quantification revealed 49% yield for M1234-anxA5-DTPA (a) and 60% for anxA5-DTPA 6 (b) by dividing band 2 (DTPA derivatives) by the sum of all three bands. cys-anxA5 dimers resulting from thiol oxidation (70 kDa) are also visible (band 3).