Therapeutic application of CCK2R-targeting PP-F11: influence of particle range, activity and peptide amount
© Konijnenberg et al.; licensee Springer. 2014
Received: 26 May 2014
Accepted: 15 August 2014
Published: 30 August 2014
Targeted radionuclide therapy with high-energy beta-emitters is generally considered suboptimal to cure small tumours (<300 mg). Tumour targeting of the CCK2 receptor-binding minigastrin analogue PP-F11 was determined in a tumour-bearing mouse model at increasing peptide amounts. The optimal therapy was analysed for PP-F11 labelled with 90Y, 177Lu or 213Bi, accounting for the radionuclide specific activities (SAs), the tumour absorbed doses and tumour (radio) biology.
Tumour uptake of 111In-PP-F11 was determined in nude mice bearing CCK2 receptor-transfected A431 xenografts at 1 and 4 h post-injection for escalating peptide masses of 0.03 to 15 nmol/mouse. The absorbed tumour dose was estimated, assuming comparable biodistributions of the 90Y, 177Lu or 213Bi radiolabelled peptides. The linear-quadratic (LQ) model was used to calculate the tumour control probabilities (TCP) as a function of tumour mass and growth.
Practically achievable maximum SAs for PP-F11 labelled with 90Y and 177Lu were 400 MBq 90Y/nmol and 120 MBq177Lu/nmol. Both the large elution volume from the 220 MBq 225Ac generator used and reaction kinetics diminished the maximum achieved 213Bi SA in practice: 40 MBq 213Bi/nmol. Tumour uptakes decreased rapidly with increasing peptide amounts, following a logarithmic curve with ED50 = 0.5 nmol. At 0.03 nmol peptide, the (300 mg) tumour dose was 9 Gy after 12 MBq 90Y-PP-F11, and for 111In and 177Lu, this was 1 Gy. A curative dose of 60 Gy could be achieved with a single administration of 111 MBq 90Y labelled to 0.28 nmol PP-F11 or with 4 × 17 MBq 213Bi (0.41 nmol) when its α-radiation relative biological effectiveness (RBE) was assumed to be 3.4. Repeated dosing is preferable to avoid complete tumour receptor saturation. Tumours larger than 200 mg are curable with 90Y-PP-F11; the other radionuclides perform better in smaller tumours. Furthermore, 177Lu is not optimal for curing fast-growing tumours.
Receptor saturation, specific radiopharmaceutical activities and absorbed doses in the tumour together favour therapy with the CCK2 receptor-binding peptide PP-F11 labelled with 90Y, despite its longer β-particle range in tissue, certainly for tumours larger than 300 mg. The predicted TCPs are of theoretical nature and need to be compared with the outcome of targeted radionuclide experiments.
KeywordsPreclinical radionuclide dosimetry Radiobiology Tumour cure model Specific activity CCK2 peptide receptor saturation Y-90, Lu-177 Bi-213 Minigastrin
PP-F11, a minigastrin analogue, was identified to be one of the most promising cholecystokinin 2 (CCK2) receptor-binding peptides to target medullary thyroid cancer (MTC) and small cell lung cancer lesions in a comparative search by the EU COST Action BM0607 . Low retention in the kidneys by PP-F11 could be achieved by replacement of the gastrin pentaglutamic acid sequence in minigastrins with five d-glutamic acid residues. Indeed in nude mice, 111In-PP-F11 showed low retention in the kidneys in combination with a high uptake in A431 xenografts transfected with the CCK2 receptor in comparison with other minigastrin analogues. The tumour and physiological organ uptake was measured at 1 and 4 h after injection of 370 kBq/0.03 nmol 111In-labelled PP-F11. Moreover, in vitro cell uptake experiments showed a high affinity of PP-F11 for the CCK2 receptor and a high internalisation rate into CCK2 receptor-transfected A431 cells . The next step towards clinical application of peptide receptor radionuclide therapy (PRRT) using this compound is a PRRT study in mice.
Preclinical PRRT studies are typically being performed in mice with tumours bearing an initial size of 8 to 9 mm in diameter, which corresponds to a spherical volume of 270 to 380 mm3, but due to different growth patterns, tumours in different animals may show large variation in size.
Low-energy beta-emitting radionuclides, such as 177Lu and 131I, are considered favourable to maximise the absorbed dose in tumours of this small size. For pragmatic reasons, the choice for a therapeutic radionuclide is often between the beta-emitters 90Y and 177Lu, as both are readily available. The latter choice (177Lu) seems to be optimal, as the mean beta-energy of 0.133 MeV yields a high probability of cure in the 1- to 3-mm (0.5 to 14 mm3) size range . The high-energy beta-emitter 90Y (0.933 MeV) shows optimal tumour control in the 28- to 42-mm range. Neither 213Bi nor any other α-emitter was evaluated in the paper by O'Donoghue et al., but with the availability of 225Ac generators  the short-range emitter 213Bi has become a very interesting radionuclide for PRRT as well.
The theoretical prediction of  was confirmed in preclinical studies of somatostatin receptor-mediated PRRT comparing the therapeutic effects of 177Lu-DOTA,Tyr3-octreotate (DOTAtate) and 90Y-DOTA,Tyr3-octreotide (DOTAtoc) in large and small tumours implanted in rats ,. With 90Y, the ideal tumour size for cure was found to be 3 to 9 cm2 (length × width, product of the two largest perpendicular diameters), which corresponds to spherical tumours with diameters between 20 and 34 mm and masses between 4 and 20 g. The absorbed dose to these tumours was between 48 and 60 Gy. With 177Lu, a more favourable response rate was found in rats bearing smaller tumours (size <1 cm or mass <0.5 g) compared with those bearing larger tumour masses. The absorbed dose to these small tumours was about 58 Gy.
We reasoned for the PRRT study that the ideal β-emitting radionuclide for labelling PP-F11 should be selected taking into account not only the absorbed radiation dose, but also the relation between the uptake kinetics in various sizes of tumours and the peptide amount to be injected. The specific activity achievable for each radionuclide dictates the peptide amount to be injected for a tumour radiation dose of 60 Gy; tumour cure is not always possible with the same peptide amount of 0.03 nmol (corresponding to 0.1 μg) as was used in the biodistribution study. Especially, neutron capture reactor-produced radionuclides show a limitation in the maximum achievable specific activity . The specific activity of the peptide should be optimized to achieve the highest possible absorbed dose to the tumour taking both the peptide mass with maximum receptor-mediated uptake and the maximum achievable specific activity into account. It is well known that unlabelled peptide will compete with the labelled peptide for the limited number of receptors and thereby reduce the uptake in target tissue -.
Estimation of the optimal therapy setting has been performed in this study for preclinical testing of PP-F11, assumed to be labelled with either 90Y, 177Lu or 213Bi. The absorbed dose in small tumours was determined, assuming the same biodistribution profile for all radiolabelled analogues and taking the influence of peptide amount on its pharmacokinetics into account as well as the radiation transport of the emitted spectra. The aim of this study was to determine which radionuclide is capable of reaching tumour-sterilizing absorbed radiation doses to the tumour of at least 60 Gy, as such a dose is expected to lead to a high probability of tumour cure over a wide range of tumour sizes according to the linear-quadratic (LQ) model (from 1 to 500 mm3) ,. For this purpose, the most important parameters influencing the dose-effect relation for curing tumours have to be determined. In this study, only the mean absorbed dose to the tumour was taken into account, without correction for possible microdosimetry induced enhancements of the absorbed dose effects by high linear energy transfer (LET) spectra and/or bystander effects.
The synthesis of the peptide PP-F11 has been described before by Kolenc-Peitl et al. ,. In summary, PP-F11 was synthesized using standard Fmoc-based solid-phase peptide synthesis and conjugated with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA), using one of the carboxylic groups of DOTA. The peptide structure was confirmed by mass spectrometry and NMR. The amino acid sequence of PP-F11 is DOTA-DGlu-DGlu-DGlu-DGlu-DGlu-DGlu-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2, and its molecular weight is 2,049 g/mol.
DOTA-conjugated peptides were radiolabelled with 111In by incubation with 111InCl3 (Covidien, Petten, The Netherlands) in 0.25 M ammonium acetate buffer, pH 5.0 under strict metal-free conditions. To reduce oxidation of the peptides during radiolabelling, 100 μg (10 μl) selenomethionine was added. The labelling mixture was incubated for 30 min at 95°C. After incubation, 50 mM EDTA was added to a final concentration of 5 mM. Radiochemical purity and the presence of oxidized peptide were checked on an HPLC system (1200 series LC system; Agilent Technologies, Palo Alto, CA, USA) equipped with an Alltima RP-C18 column (5 μm, 4.6 × 250 mm; Alltech, Deerfield, IL, USA) and an in-line NaI radiodetector (Raytest, Straubenhardt, Germany). A gradient from 0.1% trifluoroacetic acid in water to 0.1% trifluoroacetic acid in acetonitrile was used. The maximum specific activity of the peptide was 11 GBq/μmol.
Biodistribution and tumour targeting of the 111In-labelled PP-F11 were studied in female athymic BALB/c mice with subcutaneous tumours induced by inoculation of A431 cells. Mice were inoculated with 2 × 106 A431-CCK2R cells (0.2 ml) in the left flank and with A431 mock-transfected cells in the right flank. After approximately 10 days, when the tumours had reached a weight of approximately 100 to 200 mg, the mice were randomly divided into groups of five mice. The mice were injected intravenously with 370 kBq (0.03 nmol) of 111In-labelled peptide via the lateral tail vein. In an additional experiment, six groups of mice were injected with 370 kBq/0.05 nmol 111In-PP-F11 supplemented with an increasing amount (range: 3, 10, 30, 100, and 300 times) of molar excess of the unlabelled PP-F11 to determine the receptor saturation effect as well as the non-receptor specific uptake in CCK2 receptor-expressing tissues. Mice were killed by CO2 asphyxiation at 1 and 4 h after administration in the biodistribution study and after 2 h in the receptor saturation study. A blood sample was drawn, and organs of interest and the tumour were dissected, weighed and counted in a gamma counter. The animal experiments were approved by the local animal welfare committee and performed according to national regulations.
Tumour absorbed dose rate S-factors
Emission spectra and decay half-lives of 90 Y, 111 In, 177 Lu and 213 Bi and its daughters
Auger e- <1 keV
Electrons >1 keV
X-rays <1 keV
X-rays >1 keV
Auger e- <1 keV
Electrons >1 keV
Auger e- <1 keV
Electrons >1 keV
Auger e- <1 keV
Electrons >1 keV
The absorbed energy distribution was determined in each spherical tumour by using 25 equal-volume concentric spherical shells within the source volume and 25 equal-volume spherical shells around the tumour extending to a radial distance of 1 cm as a consistency check of the electron transport model used. Absorbed dose rate per unit activity S-factors was calculated according to the equation:where k is a conversion factor to dose, ϕ i is the absorbed fraction of emitted energy E i that is emitted with yield y i , and m is the mass of the spherical shell part. The dose to the tumour is calculated according to the MIRD scheme by the product of the cumulated activity Ã and the absorbed dose S-factor D = Ã × S = A0 × Ã × S, where A0 is the administered activity and Ã is the time-integrated activity coefficient (TIAC; formerly indicated by residence time τ) of the radioactivity in the tumour .
Influence of peptide kinetics and receptor saturation on dosimetry
The in vivo behaviour of PP-F11 labelled with 90Y, 177Lu or 213Bi was assumed to be equivalent to that of 111In-labelled PP-F11.
where [Bunsp] is the unspecific-bound concentration asymptote at high peptide mass, [Bspec] is the receptor-bound concentration, and ED50 is the peptide mass that lowers the tumour concentration by 50%. This curve gives an indication of the limited number of receptors available.
Activity for 60-Gy tumour dose
Production methods and specific activities (SA) of 90 Y, 111 In, 177 Lu and 213 Bi, both per nmol PP-F11 with 2,049 g/mol
Elution from 90Sr generator
Elution from 225Ac generator
Maximum SA (GBq/nmol)
Practical SA (MBq/nmol)
The practical achievable SA of 213Bi with peptide is much lower than after labelling with 90Y, 111In or 177Lu, due to the at least twice higher reaction volume with 213Bi. The elution volume of the generator is fixed at approximately 600 μl, dependent on the generator bed volume in the preclinical setting using a 220 MBq 225Ac generator. Generators containing higher amount of 225Ac activity have larger bed volumes and higher elution volumes ,. High labelling volume or low concentration of the labelled compounds results in poor reaction kinetics of the labelling, which can be solved by increasing the temperature or taking a longer reaction time. However, at a very high temperature, denaturation of biological materials might occur, and in the case of 213Bi, a longer reaction time will result in decay of activity. By reducing the elution volume and reaction time of 213Bi labelling, a higher SA can be achieved. Presently, the time between elution and injection in the animal is in the order of 30 min, by which already 36% of the initial 213Bi radioactivity has decayed.
Tumour control probability model
In this work, the clonogenic cell density is assumed to be 10% of the total cell density of 109/g. The following LQ model parameters were considered: α = 0.27 Gy−1, α/β = 6 Gy and Tμ = 0.5 h (squamous cells, see ). The repair half-life of 0.5 h is in the lower range for tumour sub-lethal repair half-lives .
Effect of tumour growth on TCP
In the case that it was impossible to give curative doses with the radionuclide in one administration, a treatment interval of 24 h between subsequent injections was assumed with unaltered growth in the treatment gap in between.
In mice with s.c. CCK2 receptor-expressing A431 tumours, the radiolabelled PP-F11 showed a high tumour uptake and retention (9.7 × 1.8 and 6.3 × 2.8%ID/g, 1 and 4 h p.i.) . The mono-exponential curve with a biological clearance half-life of 4.9 × 2.3 h (Figure 1B) leads to higher cumulative activities for the shorter half-life radionuclides such as 90Y and 213Bi in comparison to 177Lu. An additional clearance compartment was assumed with a clearance half-life of 150 h, being a median curve between the single exponential and the situation with just physical decay after 4 h, indicated in Figure 1C. Not only the clearance kinetics but also the uptake kinetics will influence the cumulated activity, especially for the shortest half-life radionuclide 213Bi (Figure 1B). The effect of both slow and fast uptake on the cumulated activity was considered.
Absorbed dose in tumours
S -values for 90 Y, 111 In, 177 Lu and 213 Bi with its daughters, in spheres with masses between 1 and 500 mg
Absorbed dose rate per radioactivityS-value (mGy/MBq s)
Tumour time-integrated activity coefficients (TIAC) per mass for 90 Y, 111 In, 177 Lu and 213 Bi with its daughters
TIAC per tumour mass [in s/g]
1 − Exponential
+ Fast uptake
+ Slow uptake
2 − Exponential
Mean absorbed doses per administered activity for 90 Y, 111 In, 177 Lu and 213 Bi (including daughters) labelled to 0.03 nmol PP-F11
Absorbed dose per administered activity (mGy/MBq)
213Bi + daughters
Effect of peptide mass
Higher amounts of peptide are needed to guide higher amounts of radioactivity and higher absorbed doses to the tumour. However, increasing the amount of peptide will at the same time reduce the tumour TIAC, according to the saturation curve shown in Figure 2. We postulated that the kinetics of the peptide clearance were not influenced by the saturation effect, but only the final uptake at each time-point, expressed as %IA/g.
Activities and peptide mass needed for an absorbed dose of 60 Gy in a 300-mg tumour
90Y - PP-F11
111In - PP-F11
177Lu - PP-F11
213Bi - PP-F11
N × activity (MBq)
1 × 111
4 × 20
8 × 174
6 × 47
10 × 19
N × peptide (nmol)
4 × 0.05
8 × 0.21
6 × 0.39
10 × 0.47
Relative uptake (%)
Tumour mass (mg)
Mean absorbed dose to tumour (Gy)
The TCPs for absorbed doses of 60 Gy in a 300-mg tumour are 77% for 90Y, 74% for 111In, and 48% for 177Lu when given in one fraction, according to Equation 4. With fractionated therapy, the TCP drops to 21% (90Y), 16% (111In) and 12% (177Lu), despite the same dose of 60 Gy. Both reductions in TCP are caused by the lower dose rates, either by the radionuclides' half-lives or by the fractionation scheme. The TCP for 213Bi PP-F11 depends on the relative biological effectiveness (RBE) of its α-radiation (93% of the absorbed dose), which is not clear yet. A RBE higher than 1.2 will already result in a TCP of more than 90% in all tumours below 300 mg. Previously, the RBE for cell killing by 213Bi-DOTA-octreotate has been shown to be 3.4 . If this would also be the case for 213Bi-PP-F11, 90% TCP will already be reached with 4 × 17 MBq (0.41 nmol).
The dose effect by 213Bi is not influenced by the quadratic term of the LQ model due to the fact that 95% of its absorbed dose is caused by high LET α-radiation without repairable damage. Because of the short half-life, the uptake kinetics, however, do show a large influence on the cure possibilities with 213Bi (Figure 2A). When the slow uptake kinetics (T1/2 = 16 min) are applied, the absorbed dose will be reduced by 18%, whereas for the fast uptake (T1/2 = 7 min), only a 4.2% reduction in dose is observed in comparison to the absorbed dose for instantaneous uptake. Compensation for this possible dose reduction can be obtained by administering either one additional therapy cycle or 9% more radioactivity per cycle.
Influence of tumour growth rate
Based on studies with 12 CCK2 receptor-binding analogues, the biodistribution of the CCK2 analogue PP-F11 shows a very favourable uptake in CCK2 receptor-expressing tumours and relatively low renal retention, which makes it potentially suitable for therapeutic application. This study aimed to investigate its possibilities and hurdles for successful radionuclide therapy with this peptide analogue in mice with s.c. CCK2 receptor-expressing tumours. The particle range of the selected radionuclides has an important impact on the cure capability of the radioloabelled peptide for various sizes of tumours . Since the receptor-mediated tumour uptake of radiolabelled PP-F11 shows a saturable relation with peptide mass, the maximum achievable specific activity is an important parameter in this respect. Furthermore, it has to be taken into account that the production method of radionuclides determines the amount that can be labelled to peptides. Therefore, the higher specific activities reachable with 90Y and 213Bi make both radionuclides very suitable for therapy of 200- to 300-mg tumours, using lower peptide mass and thus higher tumour uptake. Yttrium (90Y) is capable of controlling larger-sized (>200 mg) tumours, whereas 213Bi is capable of curing over the whole range of masses considered, but most optimal in the smallest sizes.
This receptor saturation effect has been observed previously with somatostatin receptor-targeted peptides, both in preclinical biodistribution studies , as in clinical use ,, and has also been observed even more prominently with bombesin analogues . The limited number of receptors influences the tumour uptake of the peptide at escalating peptide doses. Pharmacokinetic compartment modelling can be used to correct for the molar concentration and receptor binding . In this study, we used the one-site dissociation curve to describe the saturable uptake in the tumour and stomach. Saturation of tumour uptake in humans after PRRT with 177Lu-DOTA-octreotate has not been observed, whereas the receptor-mediated uptake in the liver and spleen did show a decrease by the therapeutic amount of peptide .
The α-emitter 213Bi shows ideal properties to cure small lesions, with most of its absorbed dose coming from its short half-life daughter 213Po. The RBE of 213Bi-PP-F11 is not known, but when the effects are comparable to the RBE found for 213Bi-DOTA-octreotate of 3.4 , its effect is tremendous. The absorbed dose to the normal organs such as the kidneys and stomach wall has not been considered in this evaluation, as kidney protection by blocking reuptake of the peptide can reduce the renal dose ,. For all radionuclides, radiation-induced toxicity in normal organs will form the limiting amount of radioactivity. Late kidney damage will occur after cumulative doses of >27 Gy with 90Y-DOTA-octreotide . Renal uptake of the PP-F11 peptide is hardly influenced by the injected peptide mass, contrary to the tumour uptake. Consequently, using a higher amount of peptides to increase the amount of radioactivity will lead to higher absorbed doses to the kidneys while the tumour absorbed doses are decreased due to receptor saturation. The stomach wall will show radiation-induced ulceration after 45-Gy fractionated external beam exposure . For that reason, it is most probably not realistic to administer the huge amounts of radioactivity as suggested for 111In and 177Lu in the case of single-exponential clearance (Figure 3A).
Fractionation of the therapy is necessary for the practically achievable specific activities of the radionuclides considered. Fractionation will lead to higher cumulative absorbed doses in tumours and has been shown to lead to lower probability of renal toxicity in clinical application of radionuclide therapy . Fractionation of therapy with 177Lu-DOTA-octreotate in rats with CA20948 tumours has been shown to reduce renal toxicity while maintaining the same tumour response as for the single fraction therapy . This reduction in renal toxicity was also influenced by the time interval between the fractions; renal toxicity was significantly reduced for intervals of a week or longer compared to a 1-day interval. This inter-fraction recovery of renal damage is not fully explained by the repair mechanism within the LQ model . In clinical practice, 177Lu-DOTA-octreotate is given in four fractions of 7.4 GBq with a 6-week interval, also in order to allow the bone marrow to recover .
The tumour growth rate has a large influence on the cure options using the longer half-life radionuclides. Tumours with a doubling time of less than 8 days show >50% reduction of the TCP for treatment using 90Y or 111In, and with 177Lu, this reduction sets in at doubling times below 14 days. Due to its short half-life, 213Bi is capable of controlling also fast-growing tumours. This growth effect is only of concern in preclinical studies with fast-growing tumour xenografts and hardly in the clinical setting in which tumour doubling times most often are longer than 14 days. Selective control of slower growing tumour subtypes could however induce a more aggressive type in any tumour regrowth. The concept of TCP may be a too strict criterion for cure, as for slower growing tumours, the regrowth may not be visible within the typical observation period of 2 to 3 months. A lag time without tumour growth and subsequent accelerated repopulation as observed in prolonged radiotherapy sessions has not been modelled . The effect of repopulation after radionuclide therapy needs to be investigated further as it will strongly influence the cure rates for fast-growing tumours, e.g. with a lag time of 7 to 8 days, all growth effects would diminish when the therapy is completed within 1 week. The size of the tumour may not be completely representative for the number of viable cells within this volume. Doomed cells can form a part of the tumour volume in response to the radiation therapy, waiting to be cleared or encapsulated .
The basis for the radiobiological considerations is formed by the linear-quadratic model, which assumes that the tumour cells behave as cells in culture in response to radiation. The main LQ model parameters α and β are determined from external beam experiments. Response to radionuclide-induced doses is also very dependent on the repair process of sub-lethal damage. In external beam exposure, this repair takes place immediately after the dose given in a short time. With radionuclide-induced exposure, the repair is induced during the dose delivery and therefore the half-time value with which sub-lethally damaged lesions are repaired is very critical. In this work, this repair half-time was taken as 0.5 h, which is considered to be valid in general for tumours. Changing the repair half-time to 1 h increases the TCP by 10% to 20% for the beta-emitting radionuclides. Future work on radiobiological modeling for radionuclide therapy should focus on the repair mechanism and the influence of kinetics. The clearance kinetics is a dominant factor not only in the absorbed dose to the tumour, but also in the TCP by the projected dose rate. It is therefore of great importance to determine the kinetic pattern with more detail than just the two time points as taken in this study. Fractionation of radionuclide therapy should be studied on the basis of the absorbed doses given, taking the radiation sensitivity, the repair of sub-lethal damage and recovery by repopulation into account.
Receptor-mediated uptake of the CCK2 compound PP-F11 in tumours shows a steep saturation effect with increasing amounts of peptide.
The specific activities achievable for radiolabelling peptides limit the absorbed dose in tumours, as higher activities go parallel with higher peptide amounts, which might considerably reduce the tumour uptake.
Multiple injections are preferred to obtain an absorbed tumour dose in the order of 60 Gy.
For the larger xenografts (>200 mg), 90Y is the preferable radionuclide, as its high specific activity allows the use of low peptide amounts, although it lacks the capability of curing smaller-sized tumours.
For tumour xenografts in almost the whole size range (<500 mg), 213Bi shows high TCPs, taking an enhancement by increased RBE from its α-radiation into account.
The influence of the clearance kinetics is the largest with 177Lu, whereas 213Bi is most strongly influenced by the uptake kinetics.
Ongoing growth of the tumour during the absorbed dose build-up reduces the TCP for longer half-life radionuclides and has hardly any influence on the cure options for 213Bi.
MK designed the study, did the calculations including the statistics and wrote the article. WAPB, EdeB and HSC did the chemical analyses on labelling conditions. OB, PL and PKP did the animal experiments and chemical synthesis, and OB and MdeJ came up with the original question and set up the study design. All authors read the article and contributed their part. MM helped out with editing, and at the end, all authors approved the final manuscript.
This work was part of COST Action BM0607 within Framework 7 of the European Union. Lieke Joosten is acknowledged for performing the animal experiments.
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