Characterization of fast-decaying PET radiotracers solely through LC-MS/MS of constituent radioactive and carrier isotopologues
© Shetty et al.; licensee Springer. 2013
Received: 5 November 2012
Accepted: 19 December 2012
Published: 12 January 2013
The characterization of fast-decaying radiotracers that are labeled with carbon-11 (t 1/2 = 20.38 min), including critical measurement of specific radioactivity (activity per mole at a specific time) before release for use in positron-emission tomography (PET), has relied heavily on chromatographic plus radiometric measurements, each of which may be vulnerable to significant errors. Thus, we aimed to develop a mass-specific detection method using sensitive liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) for identifying 11C-labeled tracers and for verifying their specific radioactivities.
The LC-MS/MS was tuned and set up with methods to generate and measure the product ions specific for carbon-11 species and M + 1 carrier (predominantly the carbon-13 isotopologue) in four 11C-labeled tracers. These radiotracers were synthesized and then analyzed before extensive carbon-11 decay. The peak areas of carbon-11 species and M + 1 carrier from the LC-MS/MS measurement and the calculated abundances of carbon-12 carrier and M + 1 radioactive species gave the mole fraction of carbon-11 species in each sample. This value upon multiplication with the theoretical specific radioactivity of carbon-11 gave the specific radioactivity of the radiotracer.
LC-MS/MS of each 11C-labeled tracer generated the product ion peaks for carbon-11 species and M + 1 carrier at the expected LC retention time. The intensity of the radioactive peak diminished as time elapsed and was undetectable after six half-lives of carbon-11. Measurements of radiotracer-specific radioactivity determined solely by LC-MS/MS at timed intervals gave a half-life for carbon-11 (20.43 min) in excellent agreement with the value obtained radiometrically. Additionally, the LC-MS/MS measurement gave specific radioactivity values (83 to 505 GBq/μmol) in good agreement with those from conventional radiometric methods.
11C-Labeled tracers were characterized at a fundamental level involving isolation and mass detection of extremely low-abundance carbon-11 species along with the M + 1 carrier counterpart. This LC-MS/MS method for characterizing fast-decaying radiotracers is valuable in both the development and production of PET radiopharmaceuticals.
KeywordsPET radiotracers Carbon-11 Specific radioactivity LC-MS/MS
Positron-emission tomography (PET), as an expanding biomedical research and diagnostic imaging technique, relies on the use of radiotracers labeled with a positron emitter, often either carbon-11 (t 1/2 = 20.38 min) or fluorine-18 (t 1/2 = 109.7 min) [1–3]. The short physical half-lives of these radiotracers demand rapid techniques for establishing the quality of each production batch before release. A suitable technique for this purpose must conclusively identify the radiotracer and also provide specific radioactivity (SA), the ratio of the amount of radioactive compound (GBq) to the amount of compound (mol) in radioactive and non-radioactive forms (isotopologues) at a specific measurement time. High-performance liquid chromatography (HPLC) is almost universally applied to assure radiotracer identity and to measure radiochemical purity and SA at the end of each radiotracer synthesis [4, 5]. Typically, 11C-labeled tracers are produced with only a low degree of 11C enrichment (<0.1%) , representing a SA value on the order of 100 GBq/μmol. Nevertheless, an accurate determination of SA is critical in many applications especially where the radiotracer is intended to bind to low-density protein targets, such as neurotransmitter receptors, enzymes or transporters, or in specific cases where the radiotracer is pharmacologically very potent, such as some radiotracers for dopamine, nicotinic, or opiate receptors [6–8].
The identification of a PET radiotracer with HPLC relies on comparison of its retention time (t R) measured with a radioactivity detector with that of the reference non-radioactive compound measured with another detector, often an absorbance detector. In conventional measurement of the SA of a radiotracer, the detector giving the mass response is calibrated for compound mass, and the radioactivity associated with a particular carrier mass peak is measured in a calibrated detector, invariably a dose calibrator (an ionization detector). Surrogate radioisotopes (e.g., 137Cs, 57Co) are often used to calibrate ionization detectors for measuring positron emitters. The accuracy of measurements is highly vulnerable to errors in instrument settings, and to changes in sample containers and geometries, as demonstrated in several studies with fluorine-18 [9–14]. Similar issues will pertain to shorter-lived carbon-11, although this has not been well studied . In addition, the presence of unknown inadvertent impurities in the radiotracer may compromise the mass measurements and hence the accuracy of derived SA. Hitherto, no non-radiometric method for verifying conventional HPLC-radiometric measurements of 11C-labeled tracer SA has been reported.
We recognized the potential of a highly sensitive mass spectrometric technique for developing a reliable method to characterize fast-decaying PET radiotracers. Such a method seemed practical using triple quadrupole liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) because its specificity, sensitivity, and detection range were envisaged to be adequate for measuring both the radioactive species and the carrier counterpart. Additionally, the MS/MS can generate, isolate, and measure ions specifically for the positron-emitting species in the presence of a thousandfold excess carrier. Here, we describe the successful use of sensitive LC-MS/MS alone to identify and measure t 1/2 and the SA of 11C-labeled tracers. The SA values obtained by this new method are compared with those from HPLC-radiometric methods. The LC-MS/MS method was found especially useful for verifying the validity of HPLC-radiometric methods.
HPLC-radiometric measurement of SA
Freshly prepared and formulated radiotracer solution (100 μL), contained in a syringe, was measured for radioactivity in a calibrated ionization chamber (Atomlab 300, Biodex, Shirley, NY, USA) and then injected onto HPLC (Beckman, Fullerton, CA, USA). The radiotracer was eluted isocratically (acetonitrile(aq) 10 to 100 mM HCO2NH4 or 0.1% CF3CO2H) on a reverse-phase column (Onyx, Prodigy, or Luna, Phenomenex, Torrance, CA, USA) equipped with radioactivity and absorbance detectors. The mass of the carrier in the injectate was determined from a linear calibration curve generated from injections of known amounts of authentic non-radioactive standards. The SA was calculated from the decay-corrected activity present in the same volume of injectate, as measured in the calibrated ionization chamber.
Methods for radiotracer analyses were developed on an API 5000 LC-MS/MS system (AB Sciex, Foster City, CA, USA), consisting of Shimadzu LC (Columbia, MD, USA) interfaced via electrospray with triple quadrupole MS/MS operated in positive ionization mode. The technique principally involves quantitative measurement of two ions that differ by two mass-to-charge ratio (m/z) units, one for the 11C-labeled species, denoted [11C]species, and another for the carrier, denoted [M + 1]carrier. [M + 1]carrier ions are generated from isotopologues having predominantly one carbon-13 atom or another heavy stable isotope (2H, 15N, or 17O) of very low abundance. Thus, for example, a solution of PBR28 was infused into the MS/MS, and, upon recording the product ions, compound-dependent and gas parameters were optimized for the m/z 349 [M + H]+ → 121 transition for monitoring [12C]species. An acquisition method for [11C]PBR28 was set up using the same instrument parameters and with the multiple-reaction monitoring table being edited to perform transitions, m/z 348 → 120 and m/z 350 → 122 for measuring [11C]species and [M + 1]carrier, respectively (Figure 1A).
Similarly, MS/MS was tuned with dLop, MePPEP, and (R)-rolipram involving transitions m/z 463 [M + H]+ → 252, m/z 455 [M + H]+ → 351, and m/z 276 [M + H]+ → 191, respectively. Methods were set up to acquire product ions to measure [11C]species and [M + 1]carrier in these radiotracers. The dwell time in these methods ranged between 100 and 175 ms. In each method, the possible cross-talk between transitions was verified by inserting a dummy [M + H]+ → product ion transition between those for [11C]species and [M + 1]carrier. The masses for the dummy transition differed by 5 to 10 amu from those for target ions. Each compound's product ion (Figure 1) was further verified by LC-MS n (n = 2, 3) analysis in an ion-trap mass analyzer (LCQ Deca, Thermo Scientific, San Jose, CA, USA).
Each radiotracer sample was diluted four- to tenfold, and 2 to 5 μL of solution (<500 kBq) was injected with an autosampler (n = 6 or 12) at about 5-min intervals onto LC-MS/MS. Samples of [11C]PBR28, [11C]dLop, and [11C]MePPEP were chromatographed at 40°C on a C18 column (2 × 20 mm, 3 μm; Phenomenex) with a gradient of binary solvents (A/B; 400 μL/min), where A was 10 mM MeCO2NH4 in water/acetonitrile (90:10 v/v) and B was 10 mM MeCO2NH4 in acetonitrile/water (90:10 v/v). In the analysis of [11C]PBR28, for example, the pumps ran 70% A:30% B for 0.1 min and then a gradient reaching 20% A:80% B over 1 min. After 3.5 min, the mobile-phase composition was returned to the initial condition. [11C](R)-Rolipram was chromatographed on the same column eluted with a gradient of water (A) and acetonitrile (B) (A/B; 0.2% acetic acid): 80% A:20% B for 0.1 min, reaching 20% A:80% B over 2 min.
The relative abundance of the M + 1 peak was measured by injecting a solution of PBR28 onto the LC-MS/MS setup to perform m/z 349, 350 [M + H]+ → 121, 122 (12C, M + 1) transitions and thereby to provide m/z 122 to 121 peak area ratio (%). Other compounds were similarly analyzed using respective acquisition methods edited to monitor product ions of 12C and M + 1 species. The measured abundance (%) of M + 1 species relative to 12C was used for converting M + 1 into 12C peak area for radiotracer carrier. The peak area ratio (%) of M + 2 to M + 1 was measured by injecting 40 and 400 pg of PBR28 and acquiring transitions m/z 350, 351 → 122, 123.
where (1) A * M,M+1 is the sum of the measured 11C, M]species peak area and 11C, M + 1]species area calculated from the relative abundances  of 13C, 2H, 15N, and 17O in the product ion; (2) A M+1,M is the sum of the measured [M + 1]carrier peak area and 12C]carrier area calculated from the measured M + 1 abundance in the non-radioactive tracer; and (3) SATheoretical is the theoretical SA of carbon-11 (3.413 × 1020 Bq/mol), the product of ln2/t 1/2 and Avogadro's number. The SA of the radiotracer was decay-corrected to give the SA at the end of radiosynthesis (SA0) as SAe λt , where t is the time between the end of synthesis and peak elution in LC-MS/MS and λ is 0.034 min-1 (ln2/20.38). A plot of lnSA (n = 12) versus clock time of peak elution in Prism 5.02 (GraphPad, La Jolla, CA, USA) gave the value for λ and thus t 1/2 (t 1/2 = ln2/λ).
In order to exemplify the non-radiometric LC-MS/MS method that we developed for identifying and measuring the SA of 11C-labeled tracers, we report results on four radiotracers applied in human PET studies, namely [11C]PBR28, [11C]dLop, [11C]MePPEP, and [11C](R)-rolipram (Figure 1). We illustrate the development of the procedure with the example of [11C]PBR28. In the MS/MS mode of a triple quadrupole analyzer, the [M + H]+ ion of non-radioactive PBR28 was dissociated and then one of the transitions, m/z 349 [M + H]+ → 121, suitable for monitoring [11C]species and [M + 1]carrier in [11C]PBR28 was selected, and instrument parameters were optimized. The MS/MS of PBR28 in an ion-trap analyzer also yielded the product ion, m/z 121, in high abundance. In the same mass analyzer, another dissociation (MS3) involving this target ion generated secondary product ions that were consistent with the genesis of the m/z 121 ion and thus the respective [11C]species and [M + 1]carrier ions by benzylic cleavage, as shown in Figure 1A.
LC-MS/MS of non-radioactive PBR28 showed transitions m/z 349 [M + H]+ → 121 (12C) and 350 [M + H]+ → 122 (M + 1) associated with a peak eluting at 1.61 min on a short reverse-phase LC column. The measured relative abundance (8.88%) of the m/z = 122 for M + 1 species was close to the value (8.83%) calculated for the product ion formed by the benzylic cleavage of the [M + H]+ ion of PBR28 (Figure 1A). Subsequently, the MS/MS acquisition method was edited to monitor the m/z 348 → 120 transition for [11C]species in [11C]PBR28. As expected, a similar LC-MS/MS analysis of non-radioactive PBR28 showed no peak for an m/z 348 → 120 transition. Furthermore, analysis of a fully decayed preparation of [11C]PBR28 showed no peak for this transition. Thus, carrier PBR28 did not generate a peak due to the m/z 120 ion or any other spurious peak when performing the m/z 348 → 120 transition. Moreover, a dummy transition, entered between the transitions for [11C]species and [M + 1]carrier, showed no cross-talk during the acquisition.
The possibility that coeluting carrier PBR28 might affect the ionization of [11C]PBR28 was tested by injecting high (400 pg) and low (40 pg) amounts of PBR28 into the LC-MS/MS and acquiring the product ions from [M + H]+ of the M + 1 and M + 2 species. The peak area ratio (%; mean ± standard deviation (SD); n = 3) of M + 2 to M + 1 was 6.64 ± 0.02 at 400 pg and 6.52 ± 0.09 at 40 pg of PBR28. These ratios agreed well with the calculated M + 2 to M + 1 ratio of 6.56%. Thus, excess [12C]carrier did not cause either [11C]species or [M + 1]carrier to ionize differently, and their ion currents were therefore expected to represent their respective concentrations.
LC-MS/MS methods were similarly set up for the characterization of [11C]species and [M + 1]carrier in [11C]dLop, [11C]MePPEP, and [11C](R)-rolipram preparations. Analysis of [11C]dLop showed LC peaks at t R = 2.02 min due to the product ions m/z 251 and 253 of [11C]species and [M + 1]carrier, respectively (Figure 1). [11C]MePPEP showed LC peaks at t R = 2.19 min arising from the acquisition of product ions m/z 350 and 352 of [11C]species and [M + 1]carrier, respectively. Similarly, analysis of [11C](R)-rolipram showed LC peaks at t R = 1.96 min upon acquiring the product ions m/z 190 and 192 of [11C]species and [M + 1]carrier, respectively.
The LC-MS/MS measurements of [11C]species and carrier gave the mole fraction of the [11C]species, which, upon multiplication with the theoretical SA of carbon-11, gave the SA of the radiotracer (Equation 1). In calculating the mole fraction of [11C]species, the measured peak area for [M + 1]carrier was converted into that for [12C]carrier using the previously measured [M + 1]/12C ratio in PBR28, dLop, MePPEP, or (R)-rolipram. This indirect measurement had the benefit of neatly circumventing possible saturation of the detector by [12C]carrier at the concentration of the radiotracer needed for measuring [11C]species. Also, in this method, the masses of acquired ions differed by two units, and any contribution of [11C, M + 2]species to the [M + 1]carrier abundance would therefore have been negligible.
Calculation of specific radioactivity from the peak areas of [ 11 C] species and [M + 1] carrier in radiotracers
Elution time a
M + 1
Specific radioactivity (decay-corrected) of PET radiotracers measured by LC-MS/MS and radiometric methods
265.5 ± 8.5
330.4 ± 5.8
167.0 ± 2.9
234.1 ± 3.2
227.1 ± 6.1
241.4 ± 7.0
160.1 ± 7.2
244.0 ± 11.7
82.5 ± 3.0
473.9 ± 4.8
505.0 ± 9.5
459.0 ± 5.8
Here, we demonstrated the capability of electrospray ionization (ESI)-MS/MS to ionize and isolate [M + H]+ of [11C]species and then generate and detect the product ion-bearing carbon-11. The mass-specific detection of [11C]species along with [M + 1]carrier at the expected t R allowed unambiguous identification of the radiotracer. By monitoring [M + 1]carrier, instead of the carbon-12 counterpart, we avoided saturation of the MS/MS detector during simultaneous measurement of trace concentrations of [11C]species. The measurement of both species gave the SA and t 1/2 of the radiotracer while also confirming radiotracer identity. The results from multiple analyses showed that the LC-MS/MS method is fast, accurate, and reproducible enough for characterizing low-enriched, fast-decaying radiotracers. This mass-specific detection method is suitable for validating the conventional method of characterization of PET radiotracers.
We verified that the excess carrier does not impact the ionization and detection of coeluting [11C]species in our LC-MS/MS system. The peak for [11C]species declined at an expected rate and then disappeared from the ion chromatogram as carbon-11 in the product ion decayed. Secondly, the ratio of M + 2 to M + 1 isotopologues was not altered when the radiotracer's non-labeled standard was injected at a concentration comparable to that of the carrier in the radiotracer. Also, the concentrations of [11C]species and [M + 1]carrier in radiotracers were within the dynamic range of detection of the triple quadrupole MS/MS system. Before being injected into LC-MS/MS, the radiotracer preparations were diluted adequately to avoid possible saturation of the ionization and detection systems. Furthermore, no cross-talk was observed between the [M + H]+ → product ion transition for [11C]species and that for [M + 1]carrier.
Carbon-11 decays to a stable nuclide, boron-11. This energetic decay event would instantly transform any molecule in which the carbon-11 had been incorporated. The product of carbon-11 decay would have a t R and mass different from those of the [11C]species or the [M + 1]carrier. Accordingly, the LC-MS/MS acquisition detected no product or chemical impurity from the decay of any of these PET radiotracers. The measurement showed exponential loss of mass of [11C]species as the radionuclide decayed thereby gave the same expected t 1/2 value for each radiotracer.
The radiometric method of SA measurement involves two detection systems, and both are prone to errors. For example, we observed a significant difference between two dose calibrators in recording the radioactivity of the same radiotracer preparation. The sources of error in the HPLC measurement of mass may include the weighing of standard, preparation of the calibration curve solutions, injection, chromatography, and UV detection. In contrast, the LC-MS/MS technique measures the mole fraction of [11C]species in a radiotracer similar to the isotope ratio in a compound. Thus, SA was obtained simply by entering into a spreadsheet: (1) the measured LC-MS/MS peak areas of [11C]species and [M + 1]carrier, (2) the abundance of [M + 1]carrier measured in the reference standard, (3) the area for the [11C, M + 1]species ion calculated from the natural abundance of heavy stable isotopes in [11C]species, and (4) the theoretical maximum SA of a compound labeled with one carbon-11 atom, as detailed under the ‘Calculations’ subsection.
The rapid LC-MS/MS method described here enables definitive characterization of radiotracers used in human PET imaging. A mass-specific detection method such as this is not susceptible to errors commonly associated with the use of the radio-HPLC method, as discussed. It may not be feasible to use triple quadrupole MS/MS regularly; however, it provides a wholly independent non-radiometric means for confirming identity and verifying SA of a radiotracer when needed. A less expensive and more commonly used single quadrupole MS instrument may offer the sensitivity to measure 11C]species and [M + 1]carrier by selected ion monitoring of respective [M + H]+ following ESI. However, such a less specific MS method can work only if no [M − 1]+ ion is generated during ionization. Other mass analyzers such as time-of-flight and linear ion traps may also be suitable for the characterization of PET radiotracers. Finally, the LC-MS/MS method should be applicable to all 11C-labeled tracers, except in instances where no 11C-containing product ion is obtained or where interfering ions are generated. If a labeled product ion is not available, selected ion monitoring of a precursor ion ([M + H]+) with a high-resolution MS can substitute the MS/MS method for measuring 11C]species and [M + 1]carrier. Also, a high-resolution MS is likely to resolve any interfering ions of isobaric species from the product ion of 11C]species in 11C-labeled tracers. In principle, LC-MS/MS may be used for characterizing PET radiotracers labeled with other short-lived positron emitters. Although an isotope separator has been used to identify the position of 18F in labeled 1,1,1,2-tetrafluoroethane , no parallel mass spectrometric method has yet been reported for measuring the SA of 18F-labeled tracers. The LC-MS/MS method should also be suitable for this purpose, as shown with the analysis of an 18F-labeled tracer in our preliminary report .
A simultaneous mass detection of [11C]species and [M + 1]carrier in 11C-labeled PET tracers was achieved using the LC-MS/MS technique. A single rapid analysis can verify the identity including the position of label in a 11C-labeled tracer preparation as well as provide a fully independent measure of SA. The LC-MS/MS method is a valuable adjunct to other techniques used in the quality control of PET radiopharmaceuticals.
- [11C]species :
- [M + 1]carrier :
13C, 2H, 15N, or 17O carrier isotopologue
high-performance liquid chromatography
liquid chromatography-mass spectrometry/mass spectrometry
- t R :
HPLC or LC retention time.
This research was supported by the Intramural Research Program of the National Institutes of Health (NIMH). We thank our colleagues for their helpful suggestions.
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